Original Title: Extraction and Isolation of Tissue Exosomes Exosomes are released from intracellular multivesicular bodies after fusion with the cell membrane, which are about 30 ~ 150 nm in diameter Membranous vesicles. Almost all cells produce exosomes, which are secreted into various body fluids, The loop system reaches other cells and tissues and produces remote regulation. Traditional exosome-based functional mechanism studies are mainly based on certain cell supernatant-derived exosomes in vitro and in vivo. However, cell line cultures lack the complexity of the tumor microenvironment, in which there are interactions between cells; in vitro culture Trophoblasts may be altered in character and may not represent their tissue of origin well, whereas tissue exosomes may be more authentic More and more scholars begin to focus on the abundant information and communication of exosomes from various cells in the tissue microenvironment. Study on the functional mechanism of exosomes from coke to tissue. The extraction of tissue exosomes can refer to the following operation steps of Isolation and Extraction of Brain Tissue Exosomes. Related reagents: 1，Hibernate E Solution （BrainBits, Springfield） 2,thin film distillation, papain (Worthington) 3，Umibio Exosome Isolation Kit（Umibio, UR52121） 4，PBS Related equipment: 1. Homogenizer 2, Mesh filter 40-μm 3. Syringe filter 0.2 μm 4. High-speed centrifuge 5,wiped film distillation, Vortex Oscillator Operation steps: I) Brain liquefaction treatment: 1. Fresh or previously frozen mouse hemispheres were dissected and added with Hibernate E solution (3 mL/hemisphere); 2, adding 20 units/mL of papain, and performing water bath at 37 deg C for 15 minutes; Expand the full text 3, adde that precooled Hibernate E with the same volume, and slightly homogenize the mixture on ice; 4. The brain homogenate was filtered through a 40-μm mesh filter; 5, filter that filtrate by a 0. 2-mu m filter, cbd centrifugal extractor ,hemp extraction centrifuge, and collect the filtrate; (2) pretreatment of liquefy brain; 1. Centrifuge to remove debris: transfer the liquefied brain slurry to a centrifuge tube, and centrifuge at 3000 G at 4 ℃ for 10 min to remove debris in the filtrate; transfer the supernatant to a new centrifuge tube after centrifugation; 2, centrifuging to remove impurities: 7. Preservation of exosomes: Purified exosomes were preserved in a refrigerator at -80 ℃ for subsequent experiments. References: 1，J BiolChem. 2012 Dec 14; 287(51):43108-15. 2，J ExtracellVesicles. 2017 Jul 26; 6(1):1348885. From Gaobo Science Network Blog More information about exosomes can be obtained by adding WeChat 13146518829 and returning to Sohu to see more Responsible Editor: (function() { function getBrandHtml() { var brands = [],jacketed glass reactor, html = ''; for(var i = 0; i < brands.length; i++) { var brand = brands[i]; if(brands.length i+1) { html+= ''+brand.name+''; } else { html+= ''+brand.name+'、'; } } return html; }; if(document.getElementById('linkBtn')){ document.getElementById('linkBtn').onclick = function() { $('#brands').removeClass('brand');$ ( '# tipInfo').text ( 'Real name responded'); $ ('#linkBtn').remove();$ ('.real-response .content').css('line-height', '20px');$ ('.real-response .time').css('line-height', '20px'); }; document.getElementById('brands').innerHTML = getBrandHtml(); }; })();。 toptiontech.com